RP14

Role of probiotics on the devlopment of inflammatory bowel disease in mice

For this research project, Mrs Zhengyu DU has been recruited.

Main supervisor

Institute of Microbiology (T. Hudcovic & H. Tlaskalova)

Co-supervisor

University of Wageningen (M. Kleerebezem & J. Wells)

Duration : 36 months 

Start date : end of 2008/beginning of 2009

Objective. Prophylactic and therapeutic effect of probiotics on experimentally induced intestinal inflammation.

Approach. To study the influence of diets, microflora components, probiotics and  prebiotics on the development of UC. Using a mouse model of acute and chronic form of UC and a model of colorectal carcinoma, we will colonize germ-free animals with bacteria isolated from bioptic samples of patients with UC and/or with defined commensal bacteria and probiotics. We will evaluate the histological and immunohistochemical changes of intestinal mucosa, determine the expression of surface molecules on cells of innate immunity and determine mRNA levels for cytokines.

Deliverables.

  • Month-12 IBD models & probiotics
  • M-28 Characterization of bacteria from biopsies and effect on mucosal biomarkers
  • M-36 Publications and PhD viva